| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Howes, Madeline K (14)
|
VACCA |
2:34.66 |
2:43.44 |
8.78 |
| 2 |
Buckner, Adelaide C (16)
|
VABASS |
2:37.82 |
2:45.91 |
8.09 |
| 3 |
Mahland, Kaelyn M (14)
|
VALAC |
2:45.98 |
2:51.20 |
5.22 |
| 4 |
Thompson, Isabelle M (14)
|
VAACAC |
2:44.78 |
2:56.05 |
11.27 |
| 5 |
Pullen, Alexis L (15)
|
VASMAC |
2:56.20 |
3:02.28 |
6.08 |
| 6 |
Freid, Blair K (14)
|
VABASS |
2:58.82 |
3:03.89 |
5.07 |
| 7 |
Montini, Sofia M (13)
|
VABASS |
3:33.77 |
3:10.16 |
-23.61 |
| 8 |
Mandujano, Liliana I (14)
|
VASMAC |
3:08.46 |
3:14.05 |
5.59 |
| 9 |
Murdock, Emery N (14)
|
VASMAC |
3:35.18 |
3:34.93 |
-0.25 |
|
Murdock, Eden J (14)
|
VASMAC |
|
DQ |
- |
|
Nivens, Natalie M (14)
|
VALAC |
3:23.08 |
NS |
- |
|
Phillips, Rowena B (14)
|
VALAC |
2:37.27 |
NS |
- |
|
Richardson, Faith (13)
|
VALAC |
2:51.45 |
NS |
- |